Development of a 16S rRNA gene sequencing pipeline for the MiSeq

Patrick D. Schloss, PhD (@PatSchloss)
The Department of Microbiology & Immunology at The University of Michigan


The Small Subunit rRNA genes

  • Evolutionary chronometer
  • Ubiquitous across all of life (16S: B/A and 18S: E)
  • Highly conserved (good and bad)
  • Low rates of horizontal gene transfer
  • Most deposited gene into GenBank
  • Conserved primers allow us to target variable regions within the gene